Laboratory techniques

Results: 7358



#Item
891Molecular biology / Epigenetics / Polymerase chain reaction / Genomics / Laboratory techniques / Bisulfite sequencing / Sodium bisulfite / Methylation / Real-time polymerase chain reaction / Chemistry / Biology / Genetics

Materials and Methods DNA preparation: DNA was extracted from paraffin-embedded tissues using an extended proteinase K (Affymetrix) extraction and recovered in TE8 following ethanol precipitation. DNA was quantitated usi

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Source URL: biomarkerres.org

Language: English
892Laboratory techniques / Polymers / Polyolefins / Size-exclusion chromatography / Chemistry / Molecular biology / Chromatography

8. From left-to-right, seal the elution modules with the adhesive tape provided. Firmly rub the tape with a smooth round plastic object (like a lab marker pen) to firmly seal the elution elution wells. Quick Guide 5% SDS

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Source URL: www.sagescience.com

Language: English - Date: 2015-03-25 09:54:36
893Laboratory techniques / Polymers / Polyolefins / Size-exclusion chromatography / Chemistry / Molecular biology / Chromatography

8. From left-to-right, seal the elution modules with the adhesive tape provided. Firmly rub the tape with a smooth round plastic object (like a lab marker pen) to firmly seal the elution elution wells. Quick Guide 3% SDS

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Source URL: www.sagescience.com

Language: English - Date: 2015-03-25 09:54:34
894Polymerase chain reaction / Laboratory techniques / Biotechnology / Hoffmann-La Roche / Primer / Actin / Reverse transcription polymerase chain reaction / Fixation / Reverse transcriptase / Biology / Molecular biology / Biochemistry

Korsak, Dolashad and Silva and Saffrey (Saffrey et al. revised) Methods Animals Tissue samples for RNA analysis were obtained from male Sprague Dawley rats from a colony maintained at the Royal Free and University Colleg

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Source URL: journal.chemistrycentral.com

Language: English
895Thrips / Polymerase chain reaction / Laboratory techniques / Biotechnology / RAPD / Thripidae / Primer / Tospovirus / Restriction fragment length polymorphism / Biology / Molecular biology / Biochemistry

THRIPS AND TOSPOVIRUSES: PROCEEDINGS OF THE 7TH INTERNATIONAL SYMPOSIUM ON THYSANOPTERA 373 Molecular polymorphism among populations of Frankliniella intonsa Gyulai1 G., K. Bayar2, O. Törjék1, E. Kiss1, J. Kiss1, Z. S

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Source URL: www.ento.csiro.au

Language: English - Date: 2003-01-28 08:00:00
896Biochemistry / Polymerase chain reaction / Laboratory techniques / Real-time polymerase chain reaction / 16S ribosomal RNA / Reverse transcription polymerase chain reaction / Invitrogen / Primer / Complementary DNA / Biology / Molecular biology / Chemistry

Quantitative real-time PCR Total RNA extraction using Trizol Reagent 1. Bacterial cells (3 mL) were harvested and resuspended in 0.5 mL culture medium to which 0.5 mL of TRIzol was added and thoroughly mixed by pipetting

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Source URL: 2014.igem.org

Language: English - Date: 2014-10-08 11:47:43
897Science / Fluorescence / Dyes / Molecular biology / Laboratory techniques / Ultrafast laser spectroscopy / Photon / Microscopy / Fluorescence-lifetime imaging microscopy / Chemistry / Physics / Spectroscopy

id100-20 Single Photon Detection Module Application Note: Fluorescence Lifetime Measurement We thank you for your interest in our products. This application note explains the advantages brought by the id100-20 module (se

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Source URL: www.idquantique.com

Language: English - Date: 2015-03-23 10:17:13
898Biochemistry / Polymerase chain reaction / Laboratory techniques / Biotechnology / Hoffmann-La Roche / Pfu DNA polymerase / Variants of PCR / Biology / Molecular biology / Chemistry

Spore-PCR PCR is made directly on conidia added to the mixed reactions. The method generally shows high specificity giving a single PCR product only. The use of high fidelity DNA polymerases such as PfuX7 or Phusion is h

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Source URL: www.fgsc.net

Language: English - Date: 2014-10-15 11:24:41
899Laboratory techniques / Molecular biology / Chemistry / Xenotropic murine leukemia virus-related virus / Whittemore Peterson Institute / Judy Mikovits / Chronic fatigue syndrome / Prostate cancer / Gammaretrovirus / Biology / Polymerase chain reaction / Science

Article addendum Article addendum This manuscript has been published online, prior to printing. Once the issue is complete and page numbers have been assigned, the citation will change accordingly.

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Source URL: www.mecfs-vic.org.au

Language: English - Date: 2011-03-29 13:16:24
900Biochemistry / Polymerase chain reaction / Laboratory techniques / DNA / Buffers / TE buffer / Taq polymerase / DNA extraction / Nucleic acid methods / Biology / Chemistry / Molecular biology

Extraction of DNA from spores for PCR (M. Peñalva Lab, slightly modified by Oakley lab) 1. Collect the conidiospores from the surface of colonies using a toothpick (Fig. 1) and suspend them in 0.1 ml of breaking buffer

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Source URL: www.fgsc.net

Language: English - Date: 2014-10-15 11:24:41
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